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New In Vitro Methods

New In Vitro Methods

Binding assays and biophysical methods

One of the important stages in the PET radiotracer development is the in vitro evaluation of candidates with the aim of establishing a lead compound. For this, in our group we use known methods (e.g., receptor binding affinity assay) to screen libraries of analogues or use them with novel applications. For example, we have recently employed microscale thermophoresis (MST) method for evaluation of derivatives for binding to a-synuclein and compared the data with those obtained using thioflavin T fluorescence assay. MST was shown to be practical alternative to quantifying interactions between small molecules and protein aggregates.

 

Image taken from: Fisher et al, ACS Chem. Neurosci. 2017, 8(9), 2088
Image taken from: Fisher et al, ACS Chem. Neurosci. 2017, 8(9), 2088